Genetical metabolomics of flavonoid biosynthesis in Populus: a case study.
Identifieur interne : 003E11 ( Main/Exploration ); précédent : 003E10; suivant : 003E12Genetical metabolomics of flavonoid biosynthesis in Populus: a case study.
Auteurs : Kris Morreel [Belgique] ; Geert Goeminne ; Véronique Storme ; Lieven Sterck ; John Ralph ; Wouter Coppieters ; Peter Breyne ; Marijke Steenackers ; Michel Georges ; Eric Messens ; Wout BoerjanSource :
- The Plant journal : for cell and molecular biology [ 0960-7412 ] ; 2006.
Descripteurs français
- KwdFr :
- Biologie informatique (méthodes), Cartographie chromosomique (méthodes), Chromatographie en phase liquide à haute performance (méthodes), Flavonoïdes (biosynthèse), Flavonoïdes (composition chimique), Flavonoïdes (génétique), Gènes de plante (MeSH), Locus de caractère quantitatif (MeSH), Lod score (MeSH), Populus (composition chimique), Populus (génétique), Populus (métabolisme).
- MESH :
- biosynthèse : Flavonoïdes.
- composition chimique : Flavonoïdes, Populus.
- génétique : Flavonoïdes, Populus.
- métabolisme : Populus.
- méthodes : Biologie informatique, Cartographie chromosomique, Chromatographie en phase liquide à haute performance.
- Gènes de plante, Locus de caractère quantitatif, Lod score.
English descriptors
- KwdEn :
- Chromatography, High Pressure Liquid (methods), Chromosome Mapping (methods), Computational Biology (methods), Flavonoids (biosynthesis), Flavonoids (chemistry), Flavonoids (genetics), Genes, Plant (MeSH), Lod Score (MeSH), Populus (chemistry), Populus (genetics), Populus (metabolism), Quantitative Trait Loci (MeSH).
- MESH :
- chemical , biosynthesis : Flavonoids.
- chemical , chemistry : Flavonoids.
- chemical , genetics : Flavonoids.
- chemistry : Populus.
- genetics : Populus.
- metabolism : Populus.
- methods : Chromatography, High Pressure Liquid, Chromosome Mapping, Computational Biology.
- Genes, Plant, Lod Score, Quantitative Trait Loci.
Abstract
Genetical metabolomics [metabolite profiling combined with quantitative trait locus (QTL) analysis] has been proposed as a new tool to identify loci that control metabolite abundances. This concept was evaluated in a case study with the model tree Populus. Using HPLC, the peak abundances were analyzed of 15 closely related flavonoids present in apical tissues of two full-sib poplar families, Populus deltoides cv. S9-2 x P. nigra cv. Ghoy and P. deltoides cv. S9-2 x P. trichocarpa cv. V24, and correlation and QTL analysis were used to detect flux control points in flavonoid biosynthesis. Four robust metabolite quantitative trait loci (mQTL), associated with rate-limiting steps in flavonoid biosynthesis, were mapped. Each mQTL was involved in the flux control to one or two flavonoids. Based on the identities of the affected metabolites and the flavonoid pathway structure, a tentative function was assigned to three of these mQTL, and the corresponding candidate genes were mapped. The data indicate that the combination of metabolite profiling with QTL analysis is a valuable tool to identify control points in a complex metabolic pathway of closely related compounds.
DOI: 10.1111/j.1365-313X.2006.02786.x
PubMed: 16774647
Affiliations:
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(genetics)</term><term>Populus (metabolism)</term><term>Quantitative Trait Loci (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biologie informatique (méthodes)</term><term>Cartographie chromosomique (méthodes)</term><term>Chromatographie en phase liquide à haute performance (méthodes)</term><term>Flavonoïdes (biosynthèse)</term><term>Flavonoïdes (composition chimique)</term><term>Flavonoïdes (génétique)</term><term>Gènes de plante (MeSH)</term><term>Locus de caractère quantitatif (MeSH)</term><term>Lod score (MeSH)</term><term>Populus (composition chimique)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Flavonoids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Flavonoids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" 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This concept was evaluated in a case study with the model tree Populus. Using HPLC, the peak abundances were analyzed of 15 closely related flavonoids present in apical tissues of two full-sib poplar families, Populus deltoides cv. S9-2 x P. nigra cv. Ghoy and P. deltoides cv. S9-2 x P. trichocarpa cv. V24, and correlation and QTL analysis were used to detect flux control points in flavonoid biosynthesis. Four robust metabolite quantitative trait loci (mQTL), associated with rate-limiting steps in flavonoid biosynthesis, were mapped. Each mQTL was involved in the flux control to one or two flavonoids. Based on the identities of the affected metabolites and the flavonoid pathway structure, a tentative function was assigned to three of these mQTL, and the corresponding candidate genes were mapped. The data indicate that the combination of metabolite profiling with QTL analysis is a valuable tool to identify control points in a complex metabolic pathway of closely related compounds.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16774647</PMID><DateCompleted><Year>2006</Year><Month>09</Month><Day>25</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0960-7412</ISSN><JournalIssue CitedMedium="Print"><Volume>47</Volume><Issue>2</Issue><PubDate><Year>2006</Year><Month>Jul</Month></PubDate></JournalIssue><Title>The Plant journal : for cell and molecular biology</Title><ISOAbbreviation>Plant J</ISOAbbreviation></Journal><ArticleTitle>Genetical metabolomics of flavonoid biosynthesis in Populus: a case study.</ArticleTitle><Pagination><MedlinePgn>224-37</MedlinePgn></Pagination><Abstract><AbstractText>Genetical metabolomics [metabolite profiling combined with quantitative trait locus (QTL) analysis] has been proposed as a new tool to identify loci that control metabolite abundances. This concept was evaluated in a case study with the model tree Populus. Using HPLC, the peak abundances were analyzed of 15 closely related flavonoids present in apical tissues of two full-sib poplar families, Populus deltoides cv. S9-2 x P. nigra cv. Ghoy and P. deltoides cv. S9-2 x P. trichocarpa cv. V24, and correlation and QTL analysis were used to detect flux control points in flavonoid biosynthesis. Four robust metabolite quantitative trait loci (mQTL), associated with rate-limiting steps in flavonoid biosynthesis, were mapped. Each mQTL was involved in the flux control to one or two flavonoids. Based on the identities of the affected metabolites and the flavonoid pathway structure, a tentative function was assigned to three of these mQTL, and the corresponding candidate genes were mapped. The data indicate that the combination of metabolite profiling with QTL analysis is a valuable tool to identify control points in a complex metabolic pathway of closely related compounds.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Morreel</LastName><ForeName>Kris</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, B-9052 Gent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Goeminne</LastName><ForeName>Geert</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Storme</LastName><ForeName>Véronique</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Sterck</LastName><ForeName>Lieven</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Ralph</LastName><ForeName>John</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Coppieters</LastName><ForeName>Wouter</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Breyne</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Steenackers</LastName><ForeName>Marijke</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Georges</LastName><ForeName>Michel</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Messens</LastName><ForeName>Eric</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Boerjan</LastName><ForeName>Wout</ForeName><Initials>W</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>RR02781</GrantID><Acronym>RR</Acronym><Agency>NCRR NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>RR08438</GrantID><Acronym>RR</Acronym><Agency>NCRR NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType 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MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002874" MajorTopicYN="N">Chromosome Mapping</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019295" MajorTopicYN="N">Computational Biology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005419" MajorTopicYN="N">Flavonoids</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008126" MajorTopicYN="N">Lod Score</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040641" MajorTopicYN="Y">Quantitative Trait Loci</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>6</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>9</Month><Day>26</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>6</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16774647</ArticleId><ArticleId IdType="pii">TPJ2786</ArticleId><ArticleId IdType="doi">10.1111/j.1365-313X.2006.02786.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country><region><li>Province de Flandre-Orientale</li><li>Région flamande</li></region><settlement><li>Gand</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><noCountry><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name><name sortKey="Breyne, Peter" sort="Breyne, Peter" uniqKey="Breyne P" first="Peter" last="Breyne">Peter Breyne</name><name sortKey="Coppieters, Wouter" sort="Coppieters, Wouter" uniqKey="Coppieters W" first="Wouter" last="Coppieters">Wouter Coppieters</name><name sortKey="Georges, Michel" sort="Georges, Michel" uniqKey="Georges M" first="Michel" last="Georges">Michel Georges</name><name sortKey="Goeminne, Geert" sort="Goeminne, Geert" uniqKey="Goeminne G" first="Geert" last="Goeminne">Geert Goeminne</name><name sortKey="Messens, Eric" sort="Messens, Eric" uniqKey="Messens E" first="Eric" last="Messens">Eric Messens</name><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name><name sortKey="Steenackers, Marijke" sort="Steenackers, Marijke" uniqKey="Steenackers M" first="Marijke" last="Steenackers">Marijke Steenackers</name><name sortKey="Sterck, Lieven" sort="Sterck, Lieven" uniqKey="Sterck L" first="Lieven" last="Sterck">Lieven Sterck</name><name sortKey="Storme, Veronique" sort="Storme, Veronique" uniqKey="Storme V" first="Véronique" last="Storme">Véronique Storme</name></noCountry><country name="Belgique"><region name="Région flamande"><name sortKey="Morreel, Kris" sort="Morreel, Kris" uniqKey="Morreel K" first="Kris" last="Morreel">Kris Morreel</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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